NettetTo stain nuclei dilute the Hoechst 33342 stock solution 1:100 in PBS, or the propidium iodide dye stock solution 1:1000 in PBS, and incubate for 15 or 1 min, respectively. Wash the glass coverslips three times with PBS for 5 min and mount onto glass slides as described above. NettetThe Hoechst-compound is known to bind to DNA by intercalation and is not easily removed. Therefore, care must be taken to avoid direct contact or ingestion of the stain. 7. Storage Concentrated Hoechst stain, Hanks Balanced Salt Solution, slides and mounting medium may be stored at 2oC to 8oC or below. 8. Stability

n Method for DNA Quantitation Using Hoechst 33258 - Promega

NettetFluorescently labeled nanoparticles are widely used for evaluating their distribution in the biological environment. However, dye leakage can lead to misinterpretations of the nanoparticles’ biodistribution. To better understand the interactions of dyes and nanoparticles and their biological environment, we explored PLGA nanoparticles … NettetHoechst 33258, pentahydrate (bis-benzimide) (MW 623.96) (H3569) 10 mL 10 mg/mL (16.0 mM) solution in water Store at 2–6°C Protect from light • • When stored as … toys to pull behind boats

Hoechst 33258 solution Fluorescent DNA dye - Sigma-Aldrich

NettetWhen using Hoechst or SYTOX stains (30 minute incubation), first wash the cells in Hanks Balanced Salt Solution for three buffer exchanges prior to counterstaining. Wash the … NettetHoechst 33258 nucleic acid stain is a popular nuclear counterstain that emits blue fluorescence when bound to dsDNA. Its uses are similar to Hoechst 33342 for counterstaining, apoptosis and cell cycle studies, but Hoechst 33258 is reportedly less cell-permeant. It also has been used to detect DNA in solution between 250ng/ml to … NettetHoechst 33342 nucleic acid stain is a popular cell-permeant nuclear counterstain that emits blue fluorescence when bound to dsDNA. This dye is often used to distinguish … toys to mentally stimulate dogs