WebNov 10, 2024 · The T7-like cyanopodoviruses are frequently isolated and well represented in many oceanic metagenomic datasets, representing a ubiquitous and ecologically important viral component in the ocean [... Web2. Place Amicon 10K spin column into supplied collection tube 3. Add 500 μl of antibody in PBS to spin column. Close lid. 4. Spin for 20 mins at 14K, at 4C a. For all spins, place elbow of tube ...

Isolation and characterisation of KP34—a novel φKMV-like …

Bacteriophage T7 (or the T7 phage) is a bacteriophage, a virus that infects bacteria. It infects most strains of Escherichia coli and relies on these hosts to propagate. Bacteriophage T7 has a lytic life cycle, meaning that it destroys the cell it infects. It also possesses several properties that make it an ideal phage for … See more In a 1945 study by Demerec and Fano, T7 was used to describe one of the seven phage types (T1 to T7) that grow lytically on Escherichia coli; although all seven phages were numbered arbitrarily, phages with odd numbers, … See more The virus has complex structural symmetry, with a capsid of the phage that is icosahedral (twenty faces) with an inner diameter of 55 nm and a tail 19 nm in diameter and 28.5 … See more T7 has a life cycle of 17 min at 37˚C, i.e. the time from infection to the lysis of the host cell when new phage are released. Due to the short latent period, most physiological … See more The T7 promoter sequence is used extensively in molecular biology due to its extremely high affinity for T7 RNA polymerase and thus high level of expression. T7 has been used as a model in synthetic biology. … See more T7 grows on rough strains of Escherichia coli (i.e. those without full-length O-antigen polysaccharide on their surface) and some other enteric bacteria, but close relatives also infect smooth and even capsulated strains. E. coli is more resistant to T7 than to some other … See more The genome of phage T7 was among the first completely sequenced genomes and was published in 1983. The head of the phage particle contains the roughly 40 kbp dsDNA genome … See more Phage T7 has the simplest known DNA replisome, consisting of a helicase and primase that reside in a single polypeptide chain that forms a hexamer in the presence of DNA and ATP or dTTP. T7 DNA polymerase, assisted by E. coli thioredoxin, performs both See more WebSep 14, 2005 · Currently, our best estimates range from ∼ 3×10 6 viruses ml −1 in the deep sea 22, 23 to ∼ 10 8 viruses ml −1 in productive coastal waters. Assuming the volume of the oceans is 1.3×10 ... cold water shock therapy

Abundant and cosmopolitan lineage of cyanopodoviruses lacking …

WebFeb 16, 2011 · Bacteriophage KP34 is a novel virus belonging to the subfamily Autographivirinae lytic for extended-spectrum β-lactamase-producing Klebsiella pneumoniae strains. Its biological features, morphology, susceptibility to chemical and physical agents, burst size, host specificity and activity spectrum were determined. Teseptimavirus (synonyms T7 phage group, T7-like phages, T7-like viruses, T7likevirus) is a genus of viruses in the order Caudovirales, in the family Autographiviridae, in the subfamily Studiervirinae. Bacteria serve as the natural host, with transmission achieved through passive diffusion. There are currently 17 species in this genus, including the type species Escherichia virus T7. WebAug 29, 2013 · Although this clade contained only three clusters from the Dry Tortugas and Chesapeake Bay, it comprised a greater number of contigs (10) than the T7-like viruses (Supplementary Table S1), and a ... coldwater shoppers guide online editions